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Assay Development

Fundamentals and Practices

Wiley, J,
E-Book ( PDF mit Adobe DRM )
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Essential principles and practice of assay development
The first comprehensive, integrated treatment of the subject,Assay Development: Fundamentals and Practices covers the essentialsand techniques involved in carrying out an assay project in eithera biotechnology/drug discovery setting or a platform setting.
Rather than attempting comprehensive coverage of all assaydevelopment technologies, the book introduces the most widely usedassay development technologies and illustrates the art of assaydevelopment through a few commonly encountered biological targetsin assay development (e.g., proteases, kinases, ion channels, and Gprotein-coupled receptors). Just enough biological background forthese biological targets is provided so that the reader can followthe logics of assay development. Chapters discuss:
* The basics of assay development, including foundational conceptsand applications
* Commonly used instrumental methods for both biochemical assaysand cell-based assays
* Assay strategies for protein binding and enzymatic activity
* Cell-based assays
* High-throughput screening
An in-depth study of the now popular Caliper's off-chip kinaseassay provides an instructive, real-world example of the assaydevelopment process.


Titel: Assay Development
Autoren/Herausgeber: Ge Wu
Ausgabe: 1. Auflage

ISBN/EAN: 9780470583111

Seitenzahl: 450
Produktform: E-Book
Sprache: Englisch

Ge Wu, PhD, is Director of Assay Development at IrisInternational. He obtained his doctorate in biochemistry from UCLA,performed postdoctoral work at Harvard Medical School, and was amember of the faculty at Cornell University Medical College. Priorto joining Iris International, he worked at Merck & Co.,Caliper Technologies, Amphora Discovery, and FivePrimeTherapeutics. He is the founding Director of Assay Development andScreening at FivePrime Therapeutics. Dr. Wu is a leading figure inassay development and screening in biopharmaceutical discoveryusing primary cell assay and the author of an acclaimed review inthis subject. - Newsletter
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