Essential principles and practice of assay development
The first comprehensive, integrated treatment of the subject,Assay Development: Fundamentals and Practices covers the essentialsand techniques involved in carrying out an assay project in eithera biotechnology/drug discovery setting or a platform setting.
Rather than attempting comprehensive coverage of all assaydevelopment technologies, the book introduces the most widely usedassay development technologies and illustrates the art of assaydevelopment through a few commonly encountered biological targetsin assay development (e.g., proteases, kinases, ion channels, and Gprotein-coupled receptors). Just enough biological background forthese biological targets is provided so that the reader can followthe logics of assay development. Chapters discuss:
* The basics of assay development, including foundational conceptsand applications
* Commonly used instrumental methods for both biochemical assaysand cell-based assays
* Assay strategies for protein binding and enzymatic activity
* Cell-based assays
* High-throughput screening
An in-depth study of the now popular Caliper's off-chip kinaseassay provides an instructive, real-world example of the assaydevelopment process.