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Basic Cell Culture Protocols

Humana Press,
Buch
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Kurzbeschreibung

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side-population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis).

Details
Schlagworte

Titel: Basic Cell Culture Protocols
Autoren/Herausgeber: Cheryl D. Helgason, Cindy L. Miller (Hrsg.)
Aus der Reihe: Methods in Molecular Biology
Ausgabe: 3rd ed. 2005

ISBN/EAN: 9781588292841

Seitenzahl: 371
Format: 22,9 x 15,2 cm
Produktform: Hardcover/Gebunden
Gewicht: 704 g
Sprache: Englisch

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