Erweiterte
Suche ›

Celiac Disease

Methods and Protocols

von
Springer New York,
Buch
101,64 € Lieferbar in 2-3 Tagen

Kurzbeschreibung

This volume provides detailed molecular analysis of the associated pathways and cell types involved in Celiac Disease, and embraces new technologies such as next generation sequencing. The book is divided into three parts: Part I gives a background overview with a number of reviews to describe the history and nature of the disease, its diagnosis, the role of animal models, and study designs for investigating genetic susceptibility. Part II describes molecular techniques, including tissue culture, isolation and cloning of relevant cell types, high content analysis of biopsies, and HLA genotyping. The final part of the book begins with an outline of scripting for data management, focusing on tools that are freely available to researchers who wish to explore them. It also describes analysis pipelines for bioinformatics prediction of antigenicity, quality control and analysis of GWAS data, and transcriptome analysis by next generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and thorough, Celiac Disease: Methods and Protocols brings together novel and more traditional methods in molecular biology and immunology, in order to provide a tool-kit for all stages for Celiac Disease research—from the practicalities of identifying, obtaining, stabilizing and maintaining high quality samples, to molecular analysis and bioinformatics.

Details
Schlagworte
Autor

Titel: Celiac Disease
Autoren/Herausgeber: Anthony W. Ryan (Hrsg.)
Aus der Reihe: Methods in Molecular Biology
Ausgabe: 1st ed. 2015

ISBN/EAN: 9781493928385

Seitenzahl: 235
Format: 25,4 x 17,8 cm
Produktform: Mixed Media
Gewicht: 726 g
Sprache: Englisch

Anthony W. Ryan PhD Room 1.17, Institute of Molecular Medicine Trinity Centre for Health Sciences Trinity College Dublin St James's Hospital, Dublin 8, Ireland. tel +353 1 896 3273 (lab 896 3292) fax +353 1 454 2043 e-mail aryan12@tcd.ie

buchhandel.de - Newsletter
Möchten Sie sich für den Newsletter anmelden?


Bitte geben Sie eine gültige E-Mail-Adresse ein.
Lieber nicht