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Chemical Transformations to Explore Expanded Nucleic Acid Structure and Function

Sierke Verlag,
Buch
48,00 € Preisreferenz Lieferbar in 2-3 Tagen

Kurzbeschreibung

Nucleic acid structure and function can be expanded in the laboratory beyond the natural repertoire. A new combinatorial method is presented to identify functionally and structurally important residues in catalytic DNA. Finding new DNAs enzymes for reactions involving small organic molecules necessitates a smart design of the selection technique. In vitro selection experiments are described to identify deoxyribozymes for fluorogenic aldol condensation reactions between a modified Fischer’s aldehyde and indoleninium derivatives. Naturally occurring aldehydes were also targeted by aldol chemistry. A new bioorthogonal aldol condensation for fluorogenic labeling of 5-formylpyridmines in DNAs and RNAs was established using indoleninium salts to generate hemicyanine-type chromophores with distinct photophysical properties. Detection of 5-formylpyrimidines has been demonstrated in proof-of-principle studies, and the labeling approach is propitious for investigating natural modified DNA and RNA samples.

Details
Schlagworte

Titel: Chemical Transformations to Explore Expanded Nucleic Acid Structure and Function
Autoren/Herausgeber: Biswajit Samanta
Ausgabe: 1. Auflage

ISBN/EAN: 9783868447699

Seitenzahl: 226
Format: 21 x 14,8 cm
Produktform: Taschenbuch/Softcover
Gewicht: 300 g
Sprache: Englisch

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