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Gene Delivery to Mammalian Cells

Volume 2: Viral Gene Transfer Techniques

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Humana Press,
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Kurzbeschreibung

The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.

Details

Titel: Gene Delivery to Mammalian Cells
Autoren/Herausgeber: William C. Heiser (Hrsg.)
Aus der Reihe: Methods in Molecular Biology
Ausgabe: 2004

ISBN/EAN: 9781588290953

Seitenzahl: 566
Format: 23,5 x 15,5 cm
Produktform: Hardcover/Gebunden
Gewicht: 1,142 g
Sprache: Englisch

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