Incorporating dramatic recent advances, this textbook presents a fresh and timely introduction to modern biophysical methods. An array of new, faster and structurally higher-resolving power biophysical methods now enables scientists to examine the examination of the mysteries of life at a molecular level. So students and researchers alike need to know the technological details behind the latest methods so they can choose appropriate tools and make optimal use of them. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, Methods in Modern Biophysics employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduate and graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields.