This volume provides a wide range
of protocols used in studying the nuclear envelope, with special attention to
the experimental adjustments that may be required to successfully investigate
this complex organelle in cells from various organisms. The Nuclear Envelope:
Methods and Protocols is divided into five sections: Part I – Nuclear Envelope
Isolation; Part II – Nuclear Envelope Protein Interactions, Localization, and
Dynamics; Part III – Nuclear Envelope Interactions with the Cytoskeleton; Part
IV – Nuclear Envelope-Chromatin Interactions; and Part V – Nucleo-Cytoplasmic
Transport. Many of the modifications discussed in this book have only been
circulated within laboratories that have conducted research in this field for
many years. Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists
of the necessary materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field.